hope

A new day is coming,whether we like it or not. The question is will you control it,or will it control you?


  • Home

  • Archives

  • Code

  • Categories

  • Tags

  • Footprint

  • qRT-PCR

  • Project

  • Publish

  • SCI Figure

  • About

  • CV

  • Search

搭PacBio全长转录组便车的无重复样本RNA-seq分析

Posted on 2018-07-02 | In Bioinformatics | Comments: | Views: ℃
| Words count in article: | Reading time ≈


RNA-seq 分析是我当年入门生信的敲门砖,有参/无参的分析,当然还有现在升级的基于PacBio全长转录组的Illumina RNA-seq分析;
测序样本的重复性很重要,但总是能接到一些无重复的样本,对于他们的分析就没有像三次重复那么的顺手和多选择;

Read more »

PacBio基因组组装之MARVEL

Posted on 2018-05-01 | In Bioinformatics | Comments: | Views: ℃
| Words count in article: | Reading time ≈

1. 软件介绍

PacBio三代数据基因组组装,针对低测序深度的组装;

Read more »

HiC分析主要内容

Posted on 2018-05-01 | In Bioinformatics | Comments: | Views: ℃
| Words count in article: | Reading time ≈


Read more »

学IGV必看的初级教程

Posted on 2018-02-05 | In Bioinformatics | Comments: | Views: ℃
| Words count in article: | Reading time ≈

Integrative Genomics Viewer (IGV)作为一个高性能的可视化工具,可以交互式的察看综合的基因组相关数据,也友好的支持多种数据类型,自然是生信工作者必须使用的利器之一。官网也提供了很详细的使用讲解,这里仅是根据我目前需要学习摘录部分做的整理,后面有时间在做其他整理。

Read more »

A Beginner’s Guide to Learn R Programming

Posted on 2017-12-15 | In R | Comments: | Views: ℃
| Words count in article: | Reading time ≈

Author: hope @Huazhong Agricultural University


Read more »

Oxford Nanopore Sequencing

Posted on 2017-10-21 | In Bioinformatics | Comments: | Views: ℃
| Words count in article: | Reading time ≈

纳米孔测序技术

纳米孔测序技术(又称第四代测序技术)是最近几年兴起的新一代测序技术。目前测序长度可以达到150kb;

Read more »

RNAi介导的抗虫效应

Posted on 2017-09-04 | In molecular biology | Comments: | Views: ℃
| Words count in article: | Reading time ≈

在植物中表达dsRNA来达到抗虫的策略起始于陈晓亚院士的棉花中表达棉铃虫细胞色素单氧酶P450基因(CYP6AE14 )的dsRNA ,削弱幼虫对棉酚抗性【Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol】,之后不同学者将其运用于实验中(【A transgenic strategy for controlling plant bugs (Adelphocoris suturalis) through expression of double-stranded RNA homologous to fatty acyl-coenzyme A reductase in cotton】)。

Read more »

你真的懂Illumina数据质量控制吗?

Posted on 2017-09-04 | In Bioinformatics | Comments: | Views: ℃
| Words count in article: | Reading time ≈


Read more »

Circos安装和使用

Posted on 2017-05-29 | In Bioinformatics | Comments: | Views: ℃
| Words count in article: | Reading time ≈

安装主页

安装主页
已发表文章circos图(http://circos.ca/in_literature/scientific/)
教程和数据下载
Creating Circos Plots

Read more »

单细胞扩增之:LIANTI

Posted on 2017-05-28 | In Single-cell | Comments: | Views: ℃
| Words count in article: | Reading time ≈

之前我们也了解过Single Cell 全基因组扩增过程,最近北大的谢老师又重新刷新了单细胞全基因组扩增的新高度:Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI),下面对本文做简单的解读?

Read more »
1234…15
tiramisutes

tiramisutes

hope bioinformatics blog
148 posts
17 categories
112 tags
RSS
GitHub E-Mail Weibo Twitter
Creative Commons
Links
  • qRT-PCR-Pipeline
  • GitBook《awk学习总结》
  • 棉花遗传改良团队
  • 生信菜鸟团-深圳大学城
  • 生信技能树
  • rabbit gao's blog
  • Huans(代谢+GWAS)
  • NSC-sequenceing
  • 伯乐在线
  • Linux开源中文社区
  • R-bloggers
  • R-resource
  • ggplot2
  • pele' blog
© 2014 – 2023 tiramisutes
Powered: Hexo
|
▽ – NexT.Muse
The total visits  times
0%